编码Ras p21的关氨酸核酸释放因子的cDNA的分子克隆
C Shou1, C L Farnsworth, B G Neel
1Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111.
Nature
|July 23, 1992
概括
研究人员确定了一种用于Ras蛋白的新型关氨酸核酸释放因子 (GRF),称为p140Ras-GRF. 这一发现促进了对Ras信号通路的理解,这些通路对细胞功能至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
- 生物化学 生物化学
背景情况:
- 细胞表面受体刺激导致活跃的,与GTP结合的Ras蛋白,这是信号转导的关键步骤.
- 抑制Ras激活会阻断受体介导的细胞功能,强调Ras的重要性.
- 拉斯激活需要GDP释放,这是一个速度限制的步骤,可能由关氨酸核酸释放因子 (GRF) 催化.
研究的目的:
- 克隆和表征一种特定于Ras蛋白的关氨酸核酸释放因子 (GRF).
- 调查已识别的Ras GRF的功能领域和特异性.
主要方法:
- 从老鼠大脑库中克隆互补的DNA.
- 序列分析以确定已知GRF的同类区域 (CDC25,Bcr,dbl).
- 在体外测试测量各种GTP结合蛋白 (RasH,RasN,Ralala,CDC42Hs) 的GDP释放.
主要成果:
- 克隆了一个编码Ras p21 (p140Ras-GRF) ~140 kDa GRF的cDNA.
- 在p140Ras-GRF的碳氧末端区域与CDC25具有相似性,并且在体外加速了RasH和RasN p21的GDP释放.
- 氨基末端区域与Bcr和dbl共享同质性,这是其他GTPases的已知GRF.
结论:
- p140Ras-GRF是一种针对Ras蛋白的新型关氨酸核酸释放因子.
- 其独特的功能领域表明它在调节Ras介导的信号转导通路方面发挥了作用.
- 了解p140Ras-GRF功能是阐明Ras控制的多种细胞过程的关键.
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