新反应由II组内核核糖酶与RNA和DNA基质一起催化
M Mörl1, I Niemer, C Schmelzer
1Institut für Genetik und Mikrobiologie, Universität München, Germany.
Cell
|September 4, 1992
概括
第二组内子表现出了显著的催化灵活性. 新的反应表明,这些内子可以直接集成到DNA中,RNA复制可能使用核酸三酸盐.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 进化生物学 进化生物学
背景情况:
- 自剪接II组内子是参与基因表达的催化RNA.
- 来自酵母线粒体COB基因的BI1内子是一个研究良好的例子.
研究的目的:
- 探索自我拼接 II 组内 bI1.1. 的新型催化活动.
- 为了研究这些反应的进化影响.
主要方法:
- 研究了 bI1 内子的线性形式的反应.
- 通过使用酸化合物键能量进行过化研究.
- 检查了单链DNA与RNA的结合.
- 评估了单链DNA基质的裂变.
主要成果:
- 使用化键能量进行转化,证明了自我拼接的第一步的逆转.
- 展示了单链DNA与RNA的结合.
- 报告了单链DNA的裂变.
- 通过RNA复制酶确定了 mononucleotide三酸盐的潜在用途.
- 建议在没有逆转录的情况下直接整合切除的内子的DNA.
结论:
- 第二组内子具有超出自我拼接的显著催化灵活性.
- 这些发现表明,生命早期内部DNA集成和RNA复制的新机制.
- 催化活动意味着II组内基因在基因组进化中的潜在作用.
相关概念视频
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...


