Jove
Visualize
联系我们

相关概念视频

PCR01:32

PCR

Overview
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Contrasting effects of Na+, K+-ATPase activation on seizure activity in acute versus chronic models.

Neuroscience·2015
Same author

Rt-PCR methods and applications.

Methods in molecular medicine·2011
Same author

Quantitative rt-PCR.

Methods in molecular medicine·2011
Same author

Quantitative rt-PCR.

Methods in molecular medicine·2011
Same author

Clinical trial of a plant-derived antibody on recolonization of mutans streptococci.

Caries research·2005
Same author

Disease profiling arrays: reverse format cDNA arrays complimentary to microarrays.

Advances in biochemical engineering/biotechnology·2004
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 5, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

有竞争力的PCR.

P D Siebert, J W Larrick

    Nature
    |October 8, 1992
    PubMed
    概括

    No abstract available in PubMed .

    更多相关视频

    Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
    07:11

    Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

    Published on: May 13, 2019

    Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
    06:54

    Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

    Published on: March 9, 2022

    相关实验视频

    Last Updated: Jul 5, 2026

    Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
    08:36

    Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

    Published on: November 1, 2018

    Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
    07:11

    Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

    Published on: May 13, 2019

    Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
    06:54

    Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

    Published on: March 9, 2022