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翻译机器和70kd热冲击蛋白在蛋白质合成中进行合作
R J Nelson1, T Ziegelhoffer, C Nicolet
1Department of Biomolecular Chemistry, University of Wisconsin-Madison 53706.
Cell
|October 2, 1992
概括
酵母热冲击蛋白 (Hsp70s) 与核糖体和新生的多相关,有助于蛋白质合成. 缺乏这些蛋白质的突变菌株表现出缓慢的生长和蛋白质合成缺陷.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 蛋白质折叠 蛋白质的折叠
背景情况:
- 热冲击蛋白 (Hsp70s) 是关键的分子伴侣,参与蛋白质平衡.
- 细胞质Hsp70s,特别是酵母中的SSB蛋白 (Ssb1/2p),与蛋白质合成有关.
- 在翻译过程中SSB Hsp70s的确切作用仍然不完全理解.
研究的目的:
- 阐明酵母SSB 70 kd热冲击蛋白 (hsp70s) 在蛋白质合成中的功能.
- 研究SSB Hsp70s与翻译核糖体和新生多的相互作用.
- 了解SSB Hsp70功能受损的生理后果.
主要方法:
- 生物化学测试以确定Ssb1/2p与翻译核糖体的关联.
- 对具有SSB基因突变的酵母菌株 (ssb1 ssb2) 的分析.
- 突变菌株的表型特征,包括生长速度和对蛋白质合成抑制剂的敏感性.
- 涉及过度表达翻译延长因子1α (EF-1α) 样基因的遗传分析.
主要成果:
- 发现SSB Hsp70s (Ssb1/2p) 与翻译核糖体有关.
- 普罗米辛治疗破坏了这种关联,表明它与新生的多结合.
- 突变的ssb1 ssb2菌株表现出缓慢的生长,减少翻译核糖体,以及对蛋白质合成抑制剂的过敏.
- 缓慢生长的表型被一种新型EF-1α类蛋白的表达增加所抑制.
结论:
- 细胞质SSB Hsp70s在协助新生的多通过核糖体中发挥着至关重要的作用.
- 这一功能类似于参与跨膜蛋白质运输的有机细胞局部化Hsp70s.
- 在酵母中,SSB Hsp70s对于高效的蛋白质合成和细胞生长至关重要.
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