用脉冲红外激光激光引起的热损伤引起的剂量依赖的光滑肌肉细胞增殖
P C Douek1, R Correa, R Neville
1National Center for Research Resources, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Md. 20892.
Circulation
|October 1, 1992
概括
血管造形术造成的热损伤,即使是短暂的,也强烈刺激了光滑肌肉细胞的增殖,这是缩的关键因素. 这项研究表明了明确的剂量反应关系,表明减少能量可以减轻这种效应,并有助于开发新的复原治疗方法.
科学领域:
- 心血管研究研究心血管研究
- 医疗器械技术 医疗器械技术
- 再生医学是一种再生医学.
背景情况:
- 使用热量 (激光,无线电频率) 的血管整形技术旨在减少剖析和反弹,但往往会导致复缩.
- 脉冲激光血管造形术用于动脉瘤切除也未能降低临床复原率.
- 区分热和机械损伤在光滑肌肉细胞 (SMC) 增殖和复原中的作用是具有挑战性的.
研究的目的:
- 为了研究热损伤在光滑肌肉细胞增殖和复原中的作用.
- 为了确定SMC扩散的大小是否可预测和剂量依赖.
- 建立一个切实可行的模型,用于选阻断缩抑制疗法.
主要方法:
- 在子的大动脉中使用脉冲的图:伊特花 (Tm:YAG) 激光诱导局部热病变.
- 通过皮肤脉冲Holmium:Yttrium Aluminum Garnet (Ho:YAG) 激光照射耳动脉,无需切除,以诱导热过渡.
- 照射后2小时至4周对动脉片段进行组织学分析,以评估急性和慢性反应.
主要成果:
- 局部热病变始终诱导着焦点SMC的扩散,在4周内填补了被切除的区域.
- 非消耗性脉冲激光辐射造成了热损伤和显著的新极限扩散 (在390mJ/mm2时缩小了59%).
- 新极端的扩散和动脉收窄显示出明显的剂量依赖于激光流动和热损伤深度.
结论:
- 动脉壁的热损伤是SMC扩散的强有力的刺激.
- 热损伤和SMC增殖反应之间存在剂量反应关系.
- 这种激光诱导的损伤模型可以用于选治疗以抑制复原.
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