系统卵子:通过与企的消化与老鼠组织快速分离
Science (New York, N.Y.)
|October 18, 1963
概括
企胺酶有效地消化 Schistosoma mansoni 感染的小鼠组织,产生大量的寄生虫卵. 这种方法通过改善寄生虫从宿主组织中恢复,有助于顺病的研究.
科学领域:
- 寄生虫学的寄生虫学
- 生物化学 生物化学
- 传染性疾病 传染性疾病
背景情况:
- 曼索尼杆菌病是一种严重的寄生虫病.
- 精确量化Schistosoma mansoni ova对于研究和诊断至关重要.
- 目前用于卵子恢复的方法可能是劳动密集型的,并产生可变的结果.
研究的目的:
- 为了评估蛋白质溶解酶企氨酸在提取Schistosoma mansoni卵中的有效性.
- 建立一种可靠的方法,从受感染的宿主组织中获得高产卵子.
主要方法:
- 来自 Schistosoma mansoni 感染小鼠的肝脏和肠道组织的同质化.
- 使用蛋白质分解酶pinguinain对均质化组织进行消化.
- 检测回收的 Schistosoma mansoni 卵子的数量.
主要成果:
- 成功获得了Schistosoma mansoni卵的高产量.
- 企的消化证明有效地从宿主组织中释放卵子.
- 该方法在寄生虫恢复方面表现出了效率.
结论:
- 企氨酸是一种强大的酶,用于在杆菌病研究中消化宿主组织.
- 这种酶性消化方法为Schistosoma mansoni卵子回收提供了一种高收益的方法.
- 这些发现支持使用企素来改善在实验环境中对寄生虫的量化.
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