酵母中的人类c-Myccoprotein的转录激活需要与Max的相互作用
B Amati1, S Dalton, M W Brooks
1Growth Control and Development of the Cell Nucleus Laboratory, Imperial Cancer Research Fund, London, UK.
Nature
|October 1, 1992
概括
Myc蛋白作为特定序列的转录激活剂,需要与Max蛋白进行二分化以进行DNA结合和基因激活. 马克斯同位体结合DNA,但不激活转录,可能对抗Myc的功能.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- c-myc 蛋白 (Myc) 具有用于转录激活和通过与 Max 蛋白 (Max) 进行二元化结合的 DNA 结合的域.
- 尽管其功能已知,但Myc和Max尚未确定为直接转录因子.
- 了解Myc在基因调节中的作用对于理解细胞过程和疾病至关重要.
研究的目的:
- 研究Myc蛋白作为特定序列的转录激活剂的直接作用.
- 为了确定Max蛋白质二元化对于Myc的DNA结合和交换能力的必要性.
- 阐明马克斯同极体与迈克斯-马克斯异极体的功能后果.
主要方法:
- 使用Saccharomyces cerevisiae作为一个体内模型系统.
- 分析Myc和Max蛋白质的DNA结合特异性.
- 评估不同二元化状态下由Myc和Max调解的转录激活.
主要成果:
- 证明Myc是特定序列的转录激活器 in vivo.
- 确认Myc的DNA结合取决于与Max的二分化.
- 表明马克斯同质二极体与Myc-Max异质二极体结合于相同的DNA位点,但缺乏交换活化潜力.
- 确定Max基本螺旋环-螺旋-氨酸拉链 (bHLH-Z) 域对Myc bHLH-Z域的亲和力比对自身更高.
结论:
- Myc作为一个直接的转录激活剂的功能,取决于与Max.的异构化.
- 马克斯同位素可以作为Myc介导的转录激活的对手.
- 在平衡状态下Myc-Max异构体的偏好形成表明了转录控制的调节机制.
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