波的细胞间传播由伊诺西三酸介导
S Boitano1, E R Dirksen, M J Sanderson
1Department of Anatomy, UCLA School of Medicine, CA 90024.
概括
伊诺西1,4,5-三酸盐 (IP3) 释放对于在呼吸道上皮细胞中传播细胞间 (Ca2+) 波至关重要. 这种信号机制涉及IP3通过间隙连接处移动,与不传播的Ca2+振荡不同.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 身体生理学 身体生理学
背景情况:
- 存在两个不同的 (Ca2+) 信号模式:传播细胞间Ca2+波和不传播细胞内Ca2+振荡 ([Ca2+]i).
- 了解区分这些Ca2+信号类型的机制对于细胞通信研究至关重要.
研究的目的:
- 研究细胞间Ca2+波与细胞内Ca2+振荡的差异传播的分子基础.
- 确定伊诺西1,4,5-三酸盐 (IP3) 信号在细胞间Ca2+波传播中的作用.
主要方法:
- 呼吸道上皮细胞用IP3受体对抗剂肝素进行治疗,使用脉冲,高频电穿孔.
- 细胞内Ca2+动态被监测在存在和缺乏肝素和thapsigargin (一个Ca2+抑制剂) 的情况下.
主要成果:
- 氨酸治疗特别抑制了细胞间Ca2+波的传播,而Ca2+振荡没有受到影响.
- 在控制条件下,Ca2+波传播发生在表现出[Ca2+]i振荡的细胞中.
- 使用thapsigargin消耗细胞内Ca2+储存,也消除了Ca2+波的传播.
结论:
- 通过伊诺西1,4,5-三酸盐 (IP3) 介导的释放是细胞间Ca2+波传播的先决条件.
- 这些发现表明,IP3可能充当信号分子,穿过间隙结以调解细胞间Ca2+波传播.
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