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相关概念视频

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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相关实验视频

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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

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马类非增殖抗体对一个哈普特基组的价值和亲属性.

N R KLIMAN, J H ROCKEY, F KARUSH

    Science (New York, N.Y.)
    |October 16, 1964
    PubMed
    概括

    研究人员从马血清中净化了一种不可沉积的抗体,发现它具有很高的亲和力和两个结合点. 这解释了为什么抗体不会沉,排除了低亲和力或非同价性作为原因.

    科学领域:

    • 免疫学 免疫学 免疫学
    • 生物化学 生物化学

    背景情况:

    • 非沉积性抗体在免疫学研究中存在挑战.
    • 了解抗体特征对于诊断和治疗至关重要.

    研究的目的:

    • 从马类血清中净化和表征一种不可沉积的抗体.
    • 为了阐明抗体不沉降性的分子基础.

    主要方法:

    • 从马类血清中净化免疫球蛋白.
    • 抗体性质的表征,包括亲和力和结合部位的静脉测量.

    主要成果:

    • 分离了具有对特定子高亲和力的A 7S beta2delta免疫球蛋白.
    • 研究人员发现,纯化的抗体每分子有两个结合点.

    结论:

    • 这种马类抗体的不沉性不是由于亲和力低或非同价性.
    • 抗体的结构和高亲和度有助于其独特的非沉行为.
    关键词:
    抗体是一种抗体.实验室研究实验室研究玛环球体中的蛋白质.这里是HAPTENS.马匹 马匹 马匹

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