Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inferring fusion nuclear burnwidths with low gain photomultiplier impulse response functions.

The Review of scientific instruments·2024
Same author

Lithium niobate waveguide squeezer with integrated cavity length stabilisation for network applications.

Optics express·2023
Same author

Publisher Correction: Burning plasma achieved in inertial fusion.

Nature·2022
Same author

Burning plasma achieved in inertial fusion.

Nature·2022
Same author

Waveguide resonator with an integrated phase modulator for second harmonic generation.

Optics express·2021
Same author

Plectin-related scapuloperoneal myopathy with treatment-responsive myasthenic syndrome.

Neuropathology and applied neurobiology·2020

相关实验视频

Updated: Jul 10, 2026

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
04:49

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Published on: September 2, 2008

从早期胚胎肌肌肉中提取出经过核核素酶处理的肌素的染色体.

E F BARIL, D S LOVE, H HERRMANN

    Science (New York, N.Y.)
    |October 16, 1964
    PubMed
    概括

    肌存在于老胚胎腿部肌肉中,但不在年轻的肌肉中. 核糖核酶治疗允许从较年轻的胚胎肌肉提取物中分离髓,揭示其存在.

    科学领域:

    • 发育生物学是发展生物学.
    • 分子生物学分子生物学
    • 生物化学 生物化学

    背景情况:

    • 肌肉蛋白是肌肉功能至关重要的蛋白质.
    • 了解肌肉蛋白的发育表达对于发育生物学和肌肉研究很重要.

    研究的目的:

    • 为了研究不同发育阶段的胚胎腿肌肉中肌素的存在和提取能力.
    • 用色谱学来确定影响髓分离的因素.

    主要方法:

    • 甲基氨基乙基纤维素色谱被用来分析腿部肌肉提取物.
    • 对比的是来自较老和11天大的小胚胎的肌肉提取物.
    • 在染色学之前,对11天大的胚胎肌肉提取物进行了核糖酶治疗.

    主要成果:

    • 从老胚胎腿部肌肉提取物中分离出三种含有髓的部分.
    • 在标准条件下,不能从11天大的胚胎肌肉提取物中化肌酸.
    • 之前的核糖核酶治疗使11天大的胚胎肌肉提取物能够从11天大的胚胎肌肉提取物中化出具有腺三酸盐酶活性的主要髓含量分数.

    结论:

    • 在的胚胎发育过程中,肌蛋白表达或可访问性发生变化.
    关键词:
    一个子胚胎染色体学 染色体学 染色体学实验室研究实验室研究肌肉蛋白质是一种肌肉蛋白质.核电复核 核电复核组织提取物

    更多相关视频

    Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
    05:59

    Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

    Published on: May 2, 2015

    A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
    10:04

    A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

    Published on: July 6, 2017

    相关实验视频

    Last Updated: Jul 10, 2026

    In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
    04:49

    In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

    Published on: September 2, 2008

    Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
    05:59

    Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos

    Published on: May 2, 2015

    A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
    10:04

    A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

    Published on: July 6, 2017

  • рибо核酶在可从早期胚胎肌肉组织中提取肌素方面发挥着作用.
  • 这表明RNA与肌肉蛋白或肌肉蛋白相关蛋白在早期发育中的潜在相互作用.