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相关概念视频

What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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相关实验视频

Updated: Jun 29, 2026

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
11:31

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

通过科尔蒂索内取消全基因抑制的废除.

K E HELLSTROEM, I HELLSTROEM, G HAUGHTON

    Science (New York, N.Y.)
    |July 2, 1965
    PubMed
    概括

    瘤细胞生长在外来老鼠杂交体中受到抑制,这种现象被称为全基抑制. 在体内和体外模型中,皮质酸治疗有效地逆转了这种生长抑制.

    科学领域:

    • 免疫学 免疫学 免疫学
    • 在瘤学瘤学.
    • 移植生物学 移植生物学

    背景情况:

    • 移植到基因不相似的主体中的瘤细胞往往表现出减少的生长.
    • 这种现象被称为异构抑制,表明由宿主因素介导的免疫反应.
    • H-2组织相容性复合体在免疫识别和排斥中起着至关重要的作用.

    研究的目的:

    • 为了研究瘤移植中异构抑制的机制.
    • 为了确定酸皮质对异性抑制的作用.
    • 探索外来异抗原在调解这种生长抑制中的作用.

    主要方法:

    • 在同基因和F (1) 杂交小鼠中进行瘤移植试验.
    • 对接受者小鼠进行皮质素酸盐的使用.
    • 瘤细胞的体外培养与含有H-2异抗原的细胞提取物.

    主要成果:

    • 在F(1) 杂交小鼠中,瘤细胞的生长比同源性小鼠减少.
    • 皮色素酸盐治疗在体内废除了异构抑制.
    • 在试验室中,当瘤细胞暴露于外来H-2异抗原时,皮酸还取消了全基性抑制.

    结论:

    关键词:
    抗原是一种抗原.科尔蒂索尼 (Cortisone) 是一种药物.实验室研究实验室研究这是遗传学.这就是ISOANTIGENS.美国的米饭.新质体免疫学 新质体免疫学新生体移植新生体移植药理学 在药理学方面.萨尔科马,实验性的组织培养 组织培养

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    • 异构抑制是限制基因异构宿主中瘤生长的重要因素.
    • 皮色酸通过调节宿主的反应,有效地克服异构抑制.
    • 外来H-2异抗原与异构抑制的机制有关.