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相关概念视频

Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

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相关实验视频

Updated: Jul 7, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

毒素残留物决定T细胞受体Vβ结合特异性

M J Irwin1, K R Hudson, J D Fraser

  • 1Department of Immunology, Scripps Research Institute, La Jolla, California 92037.

Nature
|October 29, 1992
PubMed
概括

葡萄球菌肠毒素与MHCII类结合并激活T细胞. 肠毒素中的两个关键氨基酸残留物决定了特定的T细胞受体相互作用,区分了与Vβ3和Vβ11结合的肠毒素A和E.

科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 结构生物学 结构生物学

背景情况:

  • 超抗原,像葡萄球菌肠毒素一样,通过与主要基因相容性复合体 (MHC) 类II分子和T细胞受体 (TCR) 的相互作用来参与T细胞.
  • 对于这种相互作用来说,TCRβ链至关重要,但由于混MHC相互作用,精确确定负责TCR结合的特定肠毒素区域具有挑战性.

研究的目的:

  • 为了确定葡萄球菌肠毒素中特定的氨基酸残留物,负责与不同的T细胞受体 (TCR) Vβ元素的差异性结合.
  • 阐明葡萄球菌肠毒素A和E的特异性T细胞激活特征的结构基础.

主要方法:

  • 测试葡萄球菌肠毒素A和E与可溶性TCRβ链蛋白 (Vβ3和Vβ11) 的结合.
  • 构建和分析混合性肠毒素以映射涉及TCR结合特异性的关键残留物.
  • 利用截断的肠毒素来研究T细胞刺激中的结构功能关系.

主要成果:

  • 葡萄球菌肠毒素A与Vβ3结合,而肠毒素E与Vβ11结合,尽管其序列高度相同.
  • 该研究确定了两种特定的氨基酸残留在肠毒素的碳酸终端附近作为Vβ3和Vβ11结合特异性的关键决定因素.
  • 这两种残留物负责通过特定的TCR Vβ链对肠毒素A和E的识别进行区分.

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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
11:17

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

结论:

  • 一组最小的两个氨基酸残留决定了葡萄球菌肠毒素A和E与不同的TCRVβ元素的差异性结合.
  • 这一发现提供了对超抗原介导T细胞激活和特异性背后的分子机制的关键见解.
  • 了解这些相互作用可以为开发向免疫疗法和诊断提供信息.