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Updated: Jun 10, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

免疫PCR:通过特定的抗体-DNA合物检测非常敏感的抗原

T Sano1, C L Smith, C R Cantor

  • 1Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Science (New York, N.Y.)
|October 2, 1992
PubMed
概括

一种新的免疫聚合酶连锁反应 (PCR) 试验检测到最少580个抗原分子. 这种高度敏感的抗原检测系统比现有方法有了显著的改进.

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相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.