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相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

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相关实验视频

Updated: Jul 18, 2026

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
06:23

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

Published on: January 12, 2017

膜腺三酸酶:一个数字化受体?

T Akera

    Science (New York, N.Y.)
    |November 11, 1977
    PubMed
    概括

    数字与Na+,K+-ATPase酶结合,这是一个关键受体. 这种结合与药物的作用相关,这表明的抑制导致数字化.

    科学领域:

    • 生物化学 生物化学
    • 药理学 药理学是指药理学的学科.
    • 分子生物学分子生物学

    背景情况:

    • 由于其已确定的功能相关性,Na+,K+-ATPase酶作为受体研究的模型.
    • 了解数字与这种酶的相互作用对于阐明其作用机制至关重要.

    研究的目的:

    • 为了研究数字对Na+,K+-ATPase的受体结合特性.
    • 探索数字结合及其随后的药理效应之间的关系.
    • 为了提供证据,证明抑制和数字的内作用之间的因果关系.

    主要方法:

    • 在体外研究观察数字与Na+,K+-ATPase结合.
    • 数字结亲和力和观察到的药物效应之间的相关性分析.
    • 调查其他Na+,K+-ATPase抑制剂和影响跨膜 (Na+) 运动的药物.

    主要成果:

    • 在体外与Na+,K+-ATPase结合的Digitalis符合受体结合的既定标准.
    • 在digitalis结合及其药理作用之间观察到强烈的相关性,尽管这并不是最终证明因果关系的证据.
    • 用相关抑制剂进行的进一步研究支持了这种假设,即抑制是数字的作用的基础.

    更多相关视频

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    Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

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    Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
    08:04

    Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

    Published on: November 3, 2023

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    Last Updated: Jul 18, 2026

    A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
    06:23

    A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

    Published on: January 12, 2017

    Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
    08:57

    Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

    Published on: August 18, 2017

    Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
    08:04

    Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

    Published on: November 3, 2023

    结论:

    • Na+,K+-ATPase 是一个有效的数字化受体模型.
    • 虽然相关性很高,但通过Na+,K+-ATPase抑制对数字的无otropic作用的直接因果证明需要进一步调查.
    • 证据强烈表明,阻断的,导致细胞内Na+过渡的增加,是数字无otropic效应背后的机制.