Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Electron Transport Chain01:30

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q in...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Observed psychopathology in offspring of parents with major depressive disorder, bipolar disorder and schizophrenia.

Psychological medicine·2019
Same author

Examining predictors of help-seeking behaviours in patients with mood and anxiety symptoms.

Psychiatry research·2018
Same author

Genetic testing for BRCA1 and BRCA2 in the Province of Ontario.

Clinical genetics·2015
Same author

Specific systemic lupus erythematosus disease manifestations in the six months prior to conception are associated with similar disease manifestations during pregnancy.

Lupus·2015
Same author

Development of SLE among "potential SLE" patients seen in consultation: long-term follow-up.

International journal of clinical practice·2014
Same author

[Anthropological approach to current parental perceptions of children's seizures].

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2013

相关实验视频

Updated: Jul 9, 2026

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
06:53

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Published on: November 23, 2011

牛心细胞染色体c氧化酶被多离子宏分子可逆抑制.

P PERSON, A FINE

    Science (New York, N.Y.)
    |July 1, 1960
    PubMed
    概括
    此摘要是机器生成的。

    基本蛋白质,如蛋白质胺和基因素抑制心肌细胞染色体c氧化酶. 这种抑制是可逆的聚葡萄糖硫酸盐,表明与酶的显著相互作用.

    关键词:
    心肌肌肉/新陈代谢氧化剂 / 化学

    更多相关视频

    A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
    07:14

    A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

    Published on: July 13, 2018

    Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
    10:17

    Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

    Published on: September 24, 2021

    相关实验视频

    Last Updated: Jul 9, 2026

    Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
    06:53

    Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

    Published on: November 23, 2011

    A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
    07:14

    A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

    Published on: July 13, 2018

    Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
    10:17

    Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

    Published on: September 24, 2021

    科学领域:

    • 生物化学 生物化学
    • 分子生物学分子生物学
    • 心血管科学 心血管科学

    背景情况:

    • 细胞染色体c氧化酶是线粒体电子运输链中的关键酶,对于细胞呼吸和心肌中的ATP产生至关重要.
    • 了解细胞染色体c氧化酶活性调节对于理解心脏能量代谢和潜在的治疗干预至关重要.

    研究的目的:

    • 为了研究基本蛋白对哺乳动物心肌细胞染色体c氧化酶的抑制作用.
    • 为了确定这些抑制是否可逆,并确定潜在的逆转剂.

    主要方法:

    • 使用了测量力和光谱测试来测量细胞染色体c氧化酶活性.
    • 采用了新鲜的老鼠心肌同质化物,牛心肌颗粒物 (基林和哈特里类型) 和脱氧基酸盐溶解氧化酶制剂.
    • 测试了质氨酸 (硫酸盐),基因素,酶和核糖酶的抑制作用.
    • 研究了使用强阳离子的多糖硫酸盐抑制的可逆性.

    主要成果:

    • 鉴定出原氨酸 (硫酸盐),基因素,溶酶和核糖酶是哺乳动物心肌细胞染色体c氧化酶的强有力的抑制剂.
    • 证明了由这些基本蛋白质引起的抑制通过添加多糖硫酸盐完全逆转.
    • 证实了这种抑制在心肌细胞染色体c氧化酶的不同制剂中的可逆性.

    结论:

    • 基本蛋白质可以显著调节心肌细胞染色体c氧化酶的活性.
    • 基本蛋白和细胞染色体c氧化酶之间的相互作用是可逆的,这表明一种特定的结合机制.
    • 聚氨酸化合物,如多葡萄糖硫酸盐,可以有效地抵消基本蛋白质对这种重要酶的抑制作用.