人类MHC分子呈现流感病毒的原子结构
M L Silver1, H C Guo, J L Strominger
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Nature
|November 26, 1992
概括
细胞毒性T淋巴细胞识别了由HLA-Aw68分子呈现的流感病毒. 该结构揭示了病毒片段如何与HLA-Aw68结合,形成用于T细胞受体识别的抗原表面.
科学领域:
- 免疫学 免疫学 免疫学
- 结构生物学 结构生物学
- 病毒学 病毒学
背景情况:
- 细胞毒性T淋巴细胞 (CTLs) 对于清除病毒感染的细胞至关重要.
- 在CTL上的T细胞受体 (TCR) 识别了由主要基因相容性复合体 (MHC) I类葡萄糖蛋白呈现的病毒片段.
- 了解这种相互作用的结构基础是解读免疫反应的关键.
研究的目的:
- 确定人类HLA-Aw68和流感病毒核蛋白 (Np 91-99) 之间形成的复合物的原子结构.
- 阐明与MHC I类分子结合的分子细节.
- 为了定义TCRs识别的抗原表面.
主要方法:
- 采用X射线冷晶学,确定了HLA-Aw68/流感复合物的结构.
- 结构分析的重点是结合的构造及其在MHC结合槽中的相互作用.
主要成果:
- 确定了与流感病毒核蛋白Np 91-99结合的人类I类糖蛋白HLA-Aw68的结构.
- 病毒在HLA-Aw68结合部位内采用了扩展的形状.
- 特定残留物 (P4-P8) 主要暴露,形成可识别的抗原表面.
结论:
- 该研究提供了在流感感染期间T细胞受体识别的分子复合物的详细原子视图.
- 病毒对HLA-Aw68的结合方式与结合其他MHC等位基因的内源性具有相似之处.
- 这些结构信息对于理解T细胞介导的抗病毒免疫来说至关重要.
相关概念视频
Viral Structure
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Antigen Processing Pathways
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...


