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ICAM-3的分子克隆,LFA-1的第三个配体,在静止白细胞上构成性表达
J Fawcett1, C L Holness, L A Needham
1ICRF Laboratories, University of Oxford, UK.
Nature
|December 3, 1992
概括
白细胞功能相关抗原1 (LFA-1) 与细胞间粘附分子3 (ICAM-3) 结合,这是所有白细胞上发现的蛋白质. 这种相互作用对于通过稳定细胞-细胞粘附来启动免疫反应至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 细胞表面的粘附分子稳定免疫细胞之间的相互作用.
- 白细胞功能相关抗原1 (LFA-1) 是白细胞上的一个关键受体,它与细胞间粘附分子 (ICAM) 结合.
- ICAM-1和ICAM-2是已知的LFA-1连接体,在免疫反应和白细胞对内皮的粘附中非常重要.
研究的目的:
- 为了识别和描述LFA-1,ICAM-3的第三个配体.
- 研究ICAM-3在启动免疫反应中的作用.
主要方法:
- 编码ICAM-3的互补DNA (cDNA) 的表达克隆.
- 对ICAM-3与LFA-1结合的生物化学分析.
主要成果:
- 克隆了一种补充DNA,pICAM-3,它编码了一种蛋白质,在所有白细胞上构成性表达.
- 编码的蛋白质ICAM-3与LFA-1结合.
- 在结构上,ICAM-3与ICAM-1相似,具有五个免疫球蛋白域,其结合由其两个N终端域介导.
结论:
- ICAM-3是一种新的,在白细胞上构成性表达的LFA-1联体.
- 由于其在休息白细胞上的表达,ICAM-3可能是参与启动免疫反应的主要LFA-1连接体.
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