离子与DNA结合:通过超快速时间分辨率的斯托克斯转移光谱检测
Latha A Gearheart1, Mark M Somoza, W Evan Rivers
1Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, USA.
Journal of the American Chemical Society
|September 25, 2003
概括
相反的类型显著影响DNA的内部动态. 不同于四方丁,的对应物减缓了DNA动态,影响了分子的表面极性.
科学领域:
- 生物物理化学 生物物理化学
- 分子生物学分子生物学
- 基因动力学 基因动力学
背景情况:
- 在DNA内部的动态极性影响其功能.
- 对于DNA结构和动态研究来说,了解对比效应至关重要.
研究的目的:
- 为了研究 counterions 对 DNA 内部动态极性的影响.
- 探索不同的对比因素如何影响DNA的内部分子运动.
主要方法:
- 利用时间解析的斯托克斯转移测量80ps到40ns的时间尺度.
- 采用一个极性敏感的光体 (素102) 集成到一个寡核酸序列.
主要成果:
- 随着时间的推移观察到光谱缩小与 (Na+) counterions.
- 没有显著的光谱缩小被检测到与四甲基 (TBA +) counterions.
结论:
- 同胞身份深深影响DNA的内部动态.
- 离子似乎与DNA螺旋的子集结合,阻碍它们的正常动态过程.
相关概念视频
Southern Blot
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Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Sanger Sequencing
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...


