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Updated: Aug 4, 2026

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

外共振TROSY (R1 rho - R1) 用于量化大型蛋白质中的快速交换过程

James G Kempf1, Ju-yeon Jung, Nicole S Sampson

  • 1Department of Chemistry, Yale University, P.O. Box 208107, New Haven, Connecticut 06520, USA.

Journal of the American Chemical Society
|October 2, 2003
PubMed
概括

一种新的核磁共振 (NMR) 技术扩大了对蛋白质快速运动的研究. 这项基于TROSY的实验在大型宏分子中测量了高达10^5s-1的构造性汇率.

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Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA08:17

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...