视觉色素特性在棒和圆光传导中的作用
Vladimir Kefalov1, Yingbin Fu, Nicholas Marsh-Armstrong
1Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Nature
|October 3, 2003
概括
圆和棒颜料具有类似的信号特性. 圆色素的高自发活性,而不是信号差异,解释了更快的圆反应和在昏暗光线下较低的灵敏度.
科学领域:
- 视觉科学 视觉科学 视觉科学
- 摄影传导机制的使用.
- 视网膜生理学视网膜生理学
背景情况:
- 棒和共享循环GMP光传导,但在灵敏度和动力学上有显著差异.
- 存在不同的杆状和状蛋白质变体,在颜料特性上有所不同,如meta-II状态寿命和自发异构化.
- 这些色素性质差异对杆功能分歧的功能影响仍然在很大程度上是投机性的.
研究的目的:
- 研究视觉色素特性对视网膜棒和之间的功能差异的贡献.
- 为了确定杆和圆颜料固有的信号特性是否不同.
- 为了阐明形细胞较低的光敏度和较快的动力学与杆相比背后的机制.
主要方法:
- 在Xenopus棒中表达的人类/沙兰红色圆色素.
- 在Xenopus体中表达的人类棒色素.
- 在修改后的Xenopus细胞中分析了光响应放大和动力学.
主要成果:
- 棒和色素,当表达在相同的细胞类型,引起光反应与相同的放大和动力学.
- 圆色素的自发异构化率大约是杆色素的1万倍.
- 圆色素的高自发活性被确定为适应本地圆在黑暗中的关键因素.
结论:
- 视觉色素信号特性上的差异不能解释棒和之间的功能差异.
- 圆色素的高自发异质化率有助于它们的敏感性降低和动力学加快,使圆适应白天视觉.
- 其他尚未确定的因素可能会导致棒和之间的整体功能差异.
相关概念视频
The Retina
The retina is a layer of nervous tissue at the back of the eye that transduces light into neural signals. This process, called phototransduction, is carried out by rod and cone photoreceptor cells in the back of the retina.
Vision
Vision is the result of light being detected and transduced into neural signals by the retina of the eye. This information is then further analyzed and interpreted by the brain. First, light enters the front of the eye and is focused by the cornea and lens onto the retina—a thin sheet of neural tissue lining the back of the eye. Because of refraction through the convex lens of the eye, images are projected onto the retina upside-down and reversed.
Channel Rhodopsins
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Anatomy of the Eyeball
The eye is a spherical, hollow structure composed of three tissue layers. The outer layer — the fibrous tunic, comprises the sclera — a white structure — and the cornea, which is transparent. The sclera encompasses some of the ocular surface, most of which is not visible. However, the 'white of the eye' is distinctively visible in humans compared to other species. The cornea, a clear covering at the front of the eye, enables light penetration. The eye's middle layer, the vascular tunic,...
Photoreceptors and Visual Pathways
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category, whereas...
Color Vision
Color perception begins in the retina, the light-sensitive layer at the back of the eye. Two main theories explain how colors are seen: the trichromatic theory and the opponent-process theory. The trichromatic theory, proposed by Thomas Young in 1802 and extended by Hermann von Helmholtz in 1852, suggests that color vision is based on three types of cone receptors in the retina. These cones are sensitive to different but overlapping ranges of wavelengths corresponding to red, blue, and green.


