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相关概念视频

Induced-fit Model01:13

Induced-fit Model

Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical characteristics of...
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Mechanical Protein Function01:58

Mechanical Protein Function

Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
Amino Acid Biosynthetic Pathways01:29

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which provide...

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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Published on: October 15, 2018

从皮克罗米多基酸合成酶的单模块的基质识别和道化.

Brian J Beck1, Courtney C Aldrich, Robert A Fecik

  • 1Department of Microbiology and Biotechnology Institute, University of Minnesota, Minneapolis, Minnesota 55455, USA.

Journal of the American Chemical Society
|October 9, 2003
PubMed
概括

研究人员研究了皮克罗米多基酸合成酶,揭示了链延伸机制的关键差异. 这项工作揭示了这些复杂分子是如何合成的,并提供了对多基基生物合成的见解.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 有机化学 有机化学

背景情况:

  • 聚基胺的合成涉及复杂的酶机制.
  • 皮克罗米辛 (Pik) 聚基酸合成酶在产生12和14个成员的巨乳素中是独一无二的.
  • 了解链延伸机制对于破译多基化生物合成至关重要.

研究的目的:

  • 研究皮克罗米生物合成中的链延长过程的机械细节.
  • 描述PikaIII (模块5) 和PikaIV (模块6) 的酶活性.
  • 为了比较PikAIII和PikAIV的立体化学特异性与其他多基酸合成酶系统.

主要方法:

  • 过度表达和净化PikaIII和PikaIV蛋白质.
  • 通过使用N-乙半胺激活二化物和 (14) C-甲基马洛尼尔-CoA.A 的酶活性测定.
  • 由此产生的三和四甲基乳产品的立体化学分析.

主要成果:

  • 皮卡III和皮卡IV被成功净化,并被证明可以产生三和四甲基乳.
  • 在PikAIII/PikAIV和DEBS模块之间观察到立体化学特异性的显著差异.
  • 这些发现突出了多基链延伸的基本过程中的差异.

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X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Published on: October 15, 2018

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
11:11

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11:27

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Published on: May 13, 2020

结论:

  • 这项研究阐明了皮克罗米多基酸合成酶机制的特定方面.
  • 立体化学特异性的差异表明多基基合成酶中存在明显的进化或功能适应.
  • 这项研究有助于更深入地了解控制多基胺合成的基本过程.