通过N-终端无处不在基化对p21进行蛋白质酶介导的降解
Joanna Bloom1, Virginia Amador, Francesca Bartolini
1New York University Cancer Institute and New York University School of Medicine, New York, NY 10016, USA.
Cell
|October 9, 2003
概括
细胞分裂周期调节器p21通过ubiquitin途径降解. 乌比基基提尼化发生在N-终端的氨酸,而这个部位对蛋白质体的降解至关重要.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- p21是细胞分裂周期的关键负调节剂.
- 了解p21降解对于细胞周期控制研究至关重要.
研究的目的:
- 为了阐明p21降解的机制.
- 为了确定p21蛋白质分解中涉及的特定部位和途径.
主要方法:
- 在体内和体内无处不在化试验.
- 对p21突变的分析,包括缺乏lysines的p21(K0).
- 蛋白质体降解速度的测量.
主要成果:
- p21蛋白质溶解需要功能性无素和Nedd8系统.
- p21的泛基提尼化发生在N端的甲氨酸,而不是lysines.
- 野生类型的p21和p21(K0的蛋白质分解速率相似,尽管存在不同的无处不在化水平.
结论:
- p21的蛋白质体降解是由乌比基通路调节的.
- N-终端氨酸是无处不在的关键部位,影响p21蛋白酶体能力.
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