植物对共生细菌的识别需要两个类似于LysM受体的激酶
Simona Radutoiu1, Lene Heegaard Madsen, Esben Bjørn Madsen
1Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
Nature
|October 10, 2003
概括
豆类拥有独特的基因,NFR1和NFR5,使它们能够识别根茎菌. 这些基因对最早的植物反应至关重要,促进了共生固化.
科学领域:
- 植物与微生物的相互作用
- 分子遗传学 分子遗传学
- 共生性固化的固.
背景情况:
- 豆类与根茎菌形成共生固定,这是与其他植物中的菌根共生不同的一种过程.
- 菌根和树根生物的入侵都具有早期植物遗传控制机制.
- 豆类具有独特的感知树根生物特异性信号分子的能力.
研究的目的:
- 在模型豆类Lotus japonicus中识别和表征基因,该基因负责识别细菌微共生体Mesorhizobium loti.
- 阐明这些基因在树根生物相互作用和感染的早期阶段所起的作用.
主要方法:
- 鉴定和表征两个LysM类型的氨酸/氨酸受体激酶基因,NFR1和NFR5.5.
- 对NFR1和NFR5细胞外域的分析,将它们与已知的糖和胆结合蛋白进行比较.
- 实验验证NFR1和NFR5功能在早期生理和细胞对根茎类利波基-寡糖化物信号的反应中.
主要成果:
- 在日本莲花中发现了NFR1和NFR5基因,这对于识别Mesorhizobium loti至关重要.
- 细胞外NFR1和NFR5的域表明在感知根茎类利波基-寡糖体信号方面发挥了直接作用.
- NFR1和NFR5对于植物对根茎生物信号的初始反应是不可或缺的,并确定根的感染易感性.
结论:
- NFR1和NFR5是豆类感知系统的关键组成部分,用于根茎类利波素-寡糖化物信号.
- 这些基因在通过调解早期宿主-微生物识别来启动共生固定过程中发挥着关键作用.
- 这些发现提供了关于豆类-菌体共生和宿主特异性的遗传基础的见解.
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