生物分子应激敏感仪表:通过表面介导的机械敏感膜蛋白的固定
Maryna Ornatska1, Sharon E Jones, Rajesh R Naik
1Materials Science and Engineering Department, Iowa State University, Ames, Iowa 50011, USA.
Journal of the American Chemical Society
|October 16, 2003
概括
机械敏感 (MscL) 蛋白质根据表面张力重新组织形状,从延长型转变为平坦型. 在改性表面上观察到的这种结构变化有助于应力辅助门.
科学领域:
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
- 表面化学 表面化学
背景情况:
- 机械敏感通道 (MscL) 是对机械应力有反应的关键膜蛋白.
- 了解 MscL 关机制对于细胞机械转导至关重要.
- 表面特性显著影响蛋白质的结构和功能.
研究的目的:
- 在改造的表面上研究 MscL 蛋白质的结构重组.
- 阐明表面张力和尾包装密度在 MscL gating 中的作用.
- 为了将观察到的结构变化与拟议的 MscL 关口模型相关联.
主要方法:
- 使用高分辨率原子力显微镜 (AFM) 来成像 MscL 蛋白质.
- 使用经过结末单层修改的表面.
- 操纵有机层的表面张力和尾包装密度.
主要成果:
- MscL蛋白质的形状因表面张力和尾包装而发生了巨大的变化.
- 观察到从延长型 (prolate) 到平坦型 (oblate) MscL形状的转变.
- 在高表面张力下,在平坦的MscL结构中发现了一个宽的中央开口.
结论:
- MscL的结构重组是应力辅助的,并受到表面特性的影响.
- 研究结果支持一个两阶段的"虹膜"式扩展模型.
- 表面改性为研究膜蛋白动态提供了一个平台.
相关概念视频
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:26Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.