从光激发的富勒 (C60) 中生成的活性氧物种作为潜在的药物:O2-*与1O2对比
Yoko Yamakoshi1, Naoki Umezawa, Akemi Ryu
1Division of Organic Chemistry, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158-8501, Japan. yamakosh@nihs.go.jp
Journal of the American Chemical Society
|October 16, 2003
概括
像C60这样的富勒作为光敏剂,产生反应性氧物种. 在生物条件下,这些主要包括超氧化离子激素和基激素,这些激素会导致DNA损伤.
科学领域:
- 摄影化学的使用.
- 生物化学 生物化学
- 纳米材料是一种纳米材料.
背景情况:
- 富勒 (C60和C70) 是纳米粒子,在生物系统中具有潜在的应用.
- 光敏感剂在暴露于光线时产生反应性氧物种 (ROS).
- 了解ROS生成对于评估富勒烯的安全性和有效性至关重要.
研究的目的:
- 在生物环境中描述富勒 (C60和C70) 光敏化.
- 为了确定在不同条件下由富勒产生的特定活性氧物种.
- 确定这些物种在光诱导DNA裂变中的作用.
主要方法:
- 生物化学测试 (用食尸动物进行DNA裂变).
- 物理化学方法 (EPR激素捕获,近红外光谱学).
- 化学方法 (亚蓝色四氧化物测定).
主要成果:
- 在非极性溶剂中,C60产生单点氧 (1O2).
- 在极性溶剂 (如水) 和减少剂 (如NADH) 中,C60产生超氧化离子基 (O2(-) *) 和基 (*OH) 而不是 (1O2).
- 在生理条件下,DNA裂变测试证实了O2(-) *和*OH是C60引起的光诱导DNA损伤的原因.
结论:
- 富勒烯光敏化机制在非极地和极地环境之间有显著差异.
- 在生理条件下,富勒主要产生有害的降低ROS (O2(-) **, *OH).
- 这些发现对于评估富勒伦的生物影响和治疗潜力至关重要.
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