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Updated: Aug 3, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
11:55

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

一个由光监测的α-螺旋多的侧链动态

Jean Duhamel1, Sabesh Kanagalingam, Thomas J O'Brien

  • 1Institute for Polymer Research, Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, ON, N2L 3G1, Canada.

Journal of the American Chemical Society
|October 16, 2003
PubMed
概括

用于聚合物光的blob模型成功地分析了标有pyrene标记的poly ((glutamic acid) (PGA). 这种方法揭示了蛋白质结构和动态的洞察力,可能有助于蛋白质折叠研究.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...