相关实验视频
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Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
RNA分子刺激了蛋白转化,使得蛋白转化
Nathan R Deleault1, Ralf W Lucassen, Surachai Supattapone
1Department of Biochemistry, 7200 Vail Building, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
Nature
|October 17, 2003
概括
性疾病可能需要特定的RNA分子来扩大传染性蛋白质 (PrPSc). 哺乳动物RNA刺激了这一过程,这表明宿主RNA在子病原和诊断中的作用.
科学领域:
- 神经科学是一个神经科学.
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 病与由正常的葡萄糖蛋白 (PrPC) 生成的传染性蛋白质 (PrPSc) 有关.
- 促进有效PrPSc生产的确切细胞因素尚不清楚.
- 了解PrPSc生成对于普里昂病的发病和诊断至关重要.
研究的目的:
- 为了在体外研究有效PrPSc生产所需的细胞因子.
- 探索核酸在PrPSc类蛋白 (PrPres) 的生化放大中的作用.
主要方法:
- 使用一种修改的蛋白质错折循环放大 (PMCA) 方法.
- 在体外研究了抗蛋白酶PrPSc类蛋白质 (PrPres) 的生物化学放大.
- 测试了来自不同物种的RNA制剂对PrPres放大作用.
主要成果:
- 实验室中将PrPC转化为PrPres的固体测量转换需要特定的RNA分子.
- 哺乳动物RNA制剂有效地刺激了体外PrPres放大.
- 来自无脊椎动物物种的RNA没有刺激PrPres放大.
结论:
- 宿主编码的刺激性RNA分子可能在质体疾病的发病过程中发挥作用.
- 特定的RNA分子对于有效的PrPSc in vitro放大是必不可少的.
- 这些发现提供了一种潜在的方法,可以提高对病的诊断技术的敏感性.
相关概念视频
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
03:06From DNA to Protein
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
01:26Bacterial Protein Maturation
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...