微生物合成了能量材料前体1,2,4-butanetriol的微生物合成
Wei Niu1, Mapitso N Molefe, J W Frost
1Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Journal of the American Chemical Society
|October 23, 2003
概括
微生物合成为1,2,4-butanetriol提供了一个可扩展的途径, 1,2,4-butanetriol是爆炸物的前体. 这种方法避免了恶劣的条件,与传统的化学合成不同.
科学领域:
- 生物技术和合成生物学
- 化学工程是化学工程的重要组成部分.
- 有机化学 有机化学
背景情况:
- 大规模合成1,2,4-butanetriol对于其作为替代酸甘油的应用至关重要.
- 目前生产1,2,4-butanetriol的方法无法进行工业扩展.
- 现有的合成涉及酸 (NaBH4) 减少化d,l-酸,需要高压和高温.
研究的目的:
- 为了建立d-和l-1,2,4-butanetriol的微生物合成路径.
- 为1,2,4-butanetriol生产开发可扩展的替代途径,避免恶劣的反应条件.
- 为了使1,2,4-butanetriol三酸盐能够替代酸甘油.
主要方法:
- 在微生物中设计生物合成途径.
- 使用Pseudomonas fragi. 氧化d-西洛斯到d-西隆酸.
- 使用工程Escherichia coli,将d-西隆酸转化为d-1,2,4-butanetriol.
- 使用P. fragi. 氧化l-阿拉比诺酸到l-阿拉比诺-1,4-乳酸和l-阿拉比诺酸.
- 使用工程化大肠杆菌将l-阿拉比诺酸转化为l-1,2,4-布坦醇.
主要成果:
- 在70%的产量中,d-Xylose被P. fragi. oxidized成d-xylonic酸.
- d-1,2,4-butanetriol是由大肠杆菌DH5alpha/pWN6.186A在25%的产量中从d-西隆酸中合成的.
- l-阿拉比诺酸被P. fragi. 转化为l-阿拉比诺-1,4-乳酸和l-阿拉比诺酸的混合物,产量为54%.
- l-1,2,4-butanetriol是由大肠杆菌BL21(DE3) /pWN6.222A在35%的产量中从l-阿拉比诺酸中合成的.
- 微生物合成避免了高H2压力和高温.
结论:
- 通过新的微生物通路,可以生产出纯的d-和l-1,2,4-butanetriol.
- 这种生物催化方法为传统化学合成提供了一个可扩展和更温和的替代方案.
- 开发的方法为工业应用1,2,4-butanetriol三酸盐铺平了道路.
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