循环林依赖性激酶Cdk1的目标
Jeffrey A Ubersax1, Erika L Woodbury, Phuong N Quang
1Department of Physiology, University of California, San Francisco, California 94143, USA.
Nature
|October 24, 2003
概括
研究人员确定了200个循环素依赖性激酶1 (Cdk1) 基底,揭示了细胞繁殖中的全球调节策略. 这一发现促进了对细胞循环控制机制的理解.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 细胞繁殖依赖于循环林依赖激酶 (Cdks) 的调节振荡.
- Cdks对于细胞循环控制至关重要,通过使用ATP对蛋白质基质进行酸化.
- 识别Cdk基质对于理解细胞循环调节至关重要,但很少有人知道.
研究的目的:
- 为了识别由Cdk1.1直接化的新型蛋白质基质.
- 阐明Cdk1在细胞循环控制中使用的调节机制.
主要方法:
- 一个芽的酵母蛋白质分子库的选.
- 在实验室中使用全细胞提取物进行酸化试验.
- 活体中酸化事件的验证.
主要成果:
- 确定了大约200个直接的Cdk1基质.
- 一些已识别的基质在体内显示了Cdk1依赖的酸化.
- 基质分析表明,Cdk1.1具有全球监管作用.
结论:
- Cdk1采用了一种广泛的调节策略,涉及调节分子和细胞循环机制的酸化.
- 已识别的基质为对细胞循环调节的更深入洞察提供了基础.
- 这项研究扩展了已知的Cdk1基质网络,增强了我们对细胞分裂的理解.
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...