来自动脉壁的细胞的多系潜力
Yin Tintut1, Zeni Alfonso, Trishal Saini
1Department of Medicine, David Geffen School of Medicine, UCLA, 10833 LeConte Ave, Los Angeles, Calif 90095-1679, USA. ytintut@mednet.ucla.edu
Circulation
|October 29, 2003
概括
血管细胞可以分化为多种细胞类型,类似于介质细胞干细胞. 这些细胞显示出骨,软骨和光滑肌肉发育的潜力,为动脉疾病提供了洞察力.
科学领域:
- 血管生物学 血管生物学
- 干细胞生物学 干细胞生物学
- 组织工程是组织工程.
背景情况:
- 动脉中异位组织 (骨,脂肪,软骨,髓) 的形成在糖尿病和动脉样硬化等疾病中很常见.
- 推动动脉外宫组织形成的基本机制在很大程度上是未知的.
- 一个特定的血管细胞亚群,称为化血管细胞 (CVC),来自牛大动脉中枢细胞,以前已经证明了骨质细胞分化和矿化能力.
研究的目的:
- 为了研究化血管细胞 (CVCs) 的多谱系分化潜力.
- 为了确定CVC是否具有与间酶体干细胞相似的特征.
- 探索CVCs在理解动脉内外宫组织形成方面的潜力.
主要方法:
- 对多个介质细胞系 (chondrogenic,leiomyogenic,stromogenic,adipogenic) 的评估分子和功能标记.
- 采用了细胞化学染色 (蓝) 和对II和IX类原蛋白的基因表达分析.
- 评估了光滑肌肉特异性基因表达 (α-actin,calponin,caldesmon,myosin重链) 和造血原生细胞支持能力.
主要成果:
- 通过原II/IX表达和Alcian蓝色染色,CVCs表现出原体的潜力.
- CVCs表现出leiomyogenic潜力,表达了光滑肌肉特定的标记物.
- CVCs支持了造血原生细胞的生长,表明了 stromogenic 潜力,并且在多个通道中保留了多种效能.
结论:
- 动脉壁含有具有多能分化能力的细胞,类似于介质干细胞.
- 这些来自血管的细胞具有独特的分化谱,与典型的介质干细胞不同.
- 研究结果表明,一种新的细胞机制有助于血管疾病中异位组织的形成.
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