单细胞特征对循环内皮前代细胞新血管化能力的相关性
Carmen Urbich1, Christopher Heeschen, Alexandra Aicher
1Molecular Cardiology, Department of Internal Medicine IV, University of Frankfurt, Germany.
Circulation
|October 29, 2003
概括
来自周围血液的内皮原生细胞 (EPC) 改善了缺血后的新血管化. 单细胞和非单细胞EPC都表现出类似的治疗潜力,突出了它们在再生医学中的价值.
科学领域:
- 再生医学是一种再生医学.
- 血管生物学 血管生物学
- 细胞疗法细胞疗法
背景情况:
- 循环内皮原生细胞 (EPC) 增强神经血管化后缺血.
- 目前,EPC的确切来源和特征尚不清楚.
- 研究单细胞起源在EPC功能中的作用至关重要.
研究的目的:
- 为了确定EPC表型和新血管化能力是否取决于单细胞起源.
- 为了比较来自CD14+和CD14-细胞的EPCs的治疗潜力.
- 为了评估ex vivo扩展EPCs与新分离的细胞的疗效.
主要方法:
- 从外围血液单核细胞中分离单细胞CD14+细胞.
- 用血管内皮生长因子在内皮介质中培养细胞.
- 评估内皮细胞标记物表达和殖民地形成单位.
- 将EPC移植到后肢缺血小鼠中,以评估新血管化.
主要成果:
- CD14+和CD14-衍生的EPC表现出类似的内皮标记体表达和克隆扩张能力.
- 从两个来源移植的EPC显著改善了缺血性后肢的新血管化.
- 活体扩张是必不可少的;新鲜分离的CD14+细胞没有改善新血管化.
- 不同化的巨细胞或树突细胞在促进新血管化方面不如EPC有效.
结论:
- EPCs可以从非单细胞的CD14-外周血液细胞产生.
- 在后肢缺血症后,EPC具有独特的功能活性,可以改善后肢缺血后的新血管化.
- 新血管化的治疗潜力不仅限于单细胞起源的EPC.
相关概念视频
01:19Multipotency of Hematopoietic Stem Cells
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
01:15Differentiation of Common Myeloid Progenitor Cells
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...