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Updated: Jul 18, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
09:00

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Published on: August 4, 2009

一个光激活的探针,检测细胞内蛋白激酶活性

Willem F Veldhuyzen1, Quan Nguyen, Gary McMaster

  • 1Department of Biochemistry, The Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.

Journal of the American Chemical Society
|October 30, 2003
PubMed
概括

研究人员开发了一种新型的光激活探头,用于精确测量细胞内的蛋白激酶C (PKC) 活性. 这种子传感器允许控制时间取样和检查随时间推移的酶活性.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...