Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 20, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

使用工程化细胞染色体P450 BM-3进行区域和酶选择性烯氧化.

Matthew W Peters1, Peter Meinhold, Anton Glieder

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Journal of the American Chemical Society
|October 30, 2003
PubMed
概括

工程 Bacillus 巨细胞染色体 P450 BM-3 酶选择性地氧化线性基. 这些生物催化剂在全细胞系统中表现出高活性和酶选择性.

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Development and optimization of a glycerol-based fed-batch strategy for the production of peroxidases with Komagataella phaffii under the P<sub>DF</sub> promoter.

Microbial cell factories·2026
Same author

Comprehensive assessment of fungal peroxygenases for the selective synthesis of 25-hydroxyvitamins D<sub>2</sub> and D<sub>3</sub>.

Biotechnology for biofuels and bioproducts·2026
Same author

MAPS: a marker-free genome integration system for Komagataella phaffii enabling co-production of natural pigments and industrial enzymes.

Metabolic engineering·2026
Same author

Engineering Enantiocomplementary Protoglobins for Stereoconvergent Construction of <i>N</i>-Alkylated α-Aminoketones.

Journal of the American Chemical Society·2026
Same author

Enhanced secretion of thermostable phytases from Myceliophthora thermophila by Komagataella phaffii.

Microbial cell factories·2026
Same author

A Chemoselective and Stereodivergent Platform of Heme-Nitrene Transferases to Access Chiral Aryl-β-Amino Esters and An Investigation of the Sequence-Activity Landscape.

Angewandte Chemie (International ed. in English)·2026

科学领域:

  • 生物催化剂是一种生物催化剂.
  • 酶工程是什么? 酶工程是什么?
  • 有机化学 有机化学

背景情况:

  • 来自Bacillus megaterium的细胞染色体P450 BM-3 (CYP102A1) 是一种强大的酶.
  • 为选择性基化而设计P450对于绿色化学至关重要.
  • 以前的方法往往缺乏区域和反选择性.

研究的目的:

  • 为了设计Bacillus megaterium细胞染色体P450 BM-3进行选择性烯氧化.
  • 使用大气氧来实现高的区域和酶选择性.
  • 为了证明工程P450s在全细胞生物转化中的实用性.

主要方法:

  • 使用定向进化和位点定向突变发生来修改P450 BM-3.
  • 使用工程酶变异进行了基化反应.
  • 使用表达工程P450s的大肠杆菌进行了全细胞生物转化.

主要成果:

  • 变体9-10A-A328V氧化酸转化为S-2-octanol,具有40%的反体过量 (ee).
  • 变体 1-12G 化基 (> 헥桑) 转化为 R-2 酒精,含有 40-55% ee.
  • 工程生物催化剂表现出高活性 (高达400分钟−1) 和稳定性.

结论:

  • 设计的P450 BM-3变种提供线性基的区域和酶选择性基化.
  • 这些生物催化剂在像大肠杆菌这样的高水平表达系统中保持选择性.
  • 开发的酶为生产性酒精提供了一条可持续的途径.

更多相关视频

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
13:05

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids

Published on: June 28, 2019

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes
05:57

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Published on: December 19, 2025

相关实验视频

Last Updated: Jul 20, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
13:05

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids

Published on: June 28, 2019

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes
05:57

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Published on: December 19, 2025