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相关概念视频

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Transcription Initiation01:47

Transcription Initiation

Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...

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相关实验视频

Updated: Jul 6, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

这种TATA结合蛋白和相关因素是pol III转录因子TFIIIB的组成部分.

A K Taggart1, T S Fisher, B F Pugh

  • 1Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.

Cell
|December 11, 1992
PubMed
概括
此摘要是机器生成的。

研究人员确定了新型的TATA结合蛋白相关因子 (TAFs),对于RNA聚合酶III (pol III) 转录至关重要. 这些TAFs,TAF-172和TAF-L,调节pol III活动并影响pol II转录.

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Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

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相关实验视频

Last Updated: Jul 6, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
08:51

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Published on: June 25, 2015

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

科学领域:

  • 分子生物学分子生物学
  • 基因法规 基因法规
  • 转录因子 转录因子

背景情况:

  • 塔塔结合蛋白 (TBP) 对于RNA聚合酶I,II和III的转录启动至关重要.
  • 与TBP相关的因素 (TAF) 导致转录中的聚合酶特异性.
  • 在RNA聚合酶III (pol III) 转录中TAFs的作用仍然未确立.

研究的目的:

  • 研究TAFs在pol III转录中的需求和功能.
  • 为了确定参与pol III特异转录的新型TAFs.
  • 阐明TAF赋予聚合酶特异性的机制.

主要方法:

  • 将HeLa TBP分离成素纤维素的部分.
  • 在TBP贫乏的pol II和pol III转录反应中检测TBP活性.
  • 新型TAFs,TAF-172和TAF-L.的识别和特征.

主要成果:

  • 两种新的TAFs,TAF-172和TAF-L,被确定为pol III转录的关键.
  • TAF-172通过抑制TBP与TATA盒结合来激活pol III转录,并抑制pol II转录.
  • TBP-TAF-172-TAF-L复合体在功能上取代了TFIIIB在pol III转录中的作用.

结论:

  • 波尔III转录至少涉及两个新型TAF:TAF-172和TAF-L.
  • 在赋予聚合酶特异性方面,TAFs起着至关重要的作用.
  • SL1,TFIID和TFIIIB可能代表功能上相似的TBP-TAF复合体,分别指导pol I,II和III的转录.