相关实验视频
Updated: Jul 6, 2026

07:44
High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
这种TATA结合蛋白和相关因素是pol III转录因子TFIIIB的组成部分
A K Taggart1, T S Fisher, B F Pugh
1Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Cell
|December 11, 1992
概括
研究人员确定了新型的TATA结合蛋白相关因子 (TAFs),对于RNA聚合酶III (pol III) 转录至关重要. 这些TAFs,TAF-172和TAF-L,调节pol III活动并影响pol II转录.
科学领域:
- 分子生物学分子生物学
- 基因法规 基因法规
- 转录因子 转录因子
背景情况:
- 塔塔结合蛋白 (TBP) 对于RNA聚合酶I,II和III的转录启动至关重要.
- 与TBP相关的因素 (TAF) 导致转录中的聚合酶特异性.
- 在RNA聚合酶III (pol III) 转录中TAFs的作用仍然未确立.
研究的目的:
- 研究TAFs在pol III转录中的需求和功能.
- 为了确定参与pol III特异转录的新型TAFs.
- 阐明TAF赋予聚合酶特异性的机制.
主要方法:
- 将HeLa TBP分离成素纤维素的部分.
- 在TBP贫乏的pol II和pol III转录反应中检测TBP活性.
- 新型TAFs,TAF-172和TAF-L.的识别和特征.
主要成果:
- 两种新的TAFs,TAF-172和TAF-L,被确定为pol III转录的关键.
- TAF-172通过抑制TBP与TATA盒结合来激活pol III转录,并抑制pol II转录.
- TBP-TAF-172-TAF-L复合体在功能上取代了TFIIIB在pol III转录中的作用.
结论:
- 波尔III转录至少涉及两个新型TAF:TAF-172和TAF-L.
- 在赋予聚合酶特异性方面,TAFs起着至关重要的作用.
- SL1,TFIID和TFIIIB可能代表功能上相似的TBP-TAF复合体,分别指导pol I,II和III的转录.
相关概念视频
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