罗塔病毒抗原血症和病毒血症:一个常见的事件?
Sarah E Blutt1, Carl D Kirkwood, Viviana Parreño
1Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.
Lancet (London, England)
|November 7, 2003
概括
轮状病毒可以在感染的儿童中传播到肠道之外,导致血液中可检测到轮状病毒抗原 (抗原血症). 这表明轮状病毒也可能导致病毒病,影响疾病的理解.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 罗塔病毒感染通常被认为局限于胃肠道.
- 以前的发现表明,感染儿童的脑脊髓液和血清中存在潜在的轮状病毒RNA.
- 这项研究调查了轮状病毒从肠道传播到血液中的潜力.
研究的目的:
- 为了确定轮状病毒抗原或RNA是否存在免疫能力强的儿童和轮状病毒感染的动物的血清中.
- 评估轮状病毒在胃肠道之外传播的程度.
主要方法:
- 血清从免疫能力强的小鼠,老鼠,子和小牛中收集,这些小鼠在接种后接种了罗塔病毒.
- 回顾性分析儿童血清证实轮状病毒腹,康复儿童和对照.
- 酶免疫试验 (EIA) 和逆转录聚合酶链反应 (RT-PCR) 分别用于检测轮状病毒抗原和RNA.
主要成果:
- 在受感染动物的血清和33名患有轮状病毒感染的儿童中的22名中检测到轮状病毒抗原.
- 血清抗原检测与症状出现以来的时间相反相关,直接与便抗原水平相关.
- 罗塔病毒RNA被确定在从小鼠,小牛和儿童的阳性血清样本的子集中.
结论:
- 罗塔病毒可以逃离感染儿童的胃肠道,导致抗原血症.
- 这些发现表明,儿童可能患有轮状病毒病毒病.
- 这对了解轮状病毒病原体,免疫学和临床表现具有重要意义.
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