AAA-ATPase Cdc48/p97调节了在线粒分裂结束时的线圈拆卸
Kan Cao1, Reiko Nakajima, Hemmo H Meyer
1Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, MD 21210, USA.
Cell
|November 26, 2003
概括
该AAA-ATPase Cdc48/p97伴侣复合体对于线粒状的拆卸至关重要. 这个复合体调节了微管子的动态,使细胞能够从线粒分裂过渡到相间.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 线粒体的拆解对于细胞循环的进展至关重要,但仍然不太了解.
- 众所周知,AAA-ATPase Cdc48/p97及其适配器 Ufd1-Npl4 在膜贩运中的作用.
- 对于Cdc48/p97在调节线粒体退出过程中的微管体动态的确切功能尚不清楚.
研究的目的:
- 为了研究Cdc48/p97-Ufd1-Npl4在线粒状拆解中的作用.
- 阐明Cdc48/p97-Ufd1-Npl4在线粒分裂结束时调节微管体动态的机制.
- 为了确定Cdc48/p97功能是否在各种物种中保持.
主要方法:
- 利用Xenopus卵取物在试管体内研究线索拆卸.
- 在酵母中进行基因分析,研究同类Cdc48.
- 研究了Cdc48/p97-Ufd1-Npl4与轴组装因子的直接结合.
主要成果:
- Cdc48/p97-Ufd1-Npl4对于Xenopus蛋提取物中的线粒螺旋分解是必不可少的.
- Cdc48/p97-Ufd1-Npl4功能的丧失导致持续的单极,并阻止了相间微管子阵列的形成.
- Cdc48,p97的酵母同类物,也需要用于螺旋拆卸.
- Cdc48/p97-Ufd1-Npl4 直接与组合因子相互作用,调节它们的微管相互作用.
结论:
- Cdc48/p97-Ufd1-Npl4 作为一个关键调节器的微管力学在线索性出口.
- 这种伴侣复合体对于微管的结构转变至关重要,因为细胞重新进入介相.
- 在线拆卸中Cdc48/p97的功能是从酵母保存到Xenopus.
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