阿普塔默激活了三甲染料的光
Jeremy R Babendure1, Stephen R Adams, Roger Y Tsien
1Department of Pharmacology, Howard Hughes Medical Institute, University of California-San Diego, La Jolla, CA 92093-0647, USA.
Journal of the American Chemical Society
|December 4, 2003
概括
简短的RNA序列称为aptamers可以显著提高非光染料的光. 这一发现为生物研究中新型RNA成像技术铺平了道路.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 化学生物学 化学生物学
背景情况:
- 亚胺是为特定分子结合而选择的短核酸序列.
- 三甲染料通常是非光的.
- 马六合绿 (MG) 和硫胺B (SRB) 是已知的染料.
研究的目的:
- 调查MG和SRB体增强三甲染料光的能力.
- 探索光增强的特异性和大小.
- 建立基因编码RNA成像标签的基础.
主要方法:
- 用于特定染料结合的MG和SRB体的选择.
- 光谱分析以量化光增强 (量子产量).
- 测试对不同染料的阿普坦特异性.
主要成果:
- 马氏甲酸增强了马氏甲酸和印度绿 (IMG) 的光度超过2000倍.
- SRB 体增强了蓝色 V 和 VF 的光度超过 90 倍.
- 证明了特定序列的光增强,具有最小的交叉反应性.
结论:
- MG和SRB的体可以显著增强特定非光染料的光.
- 这种序列特定的光标签是RNA动图检测的新方法.
- 代表了开发用于体外和细胞RNA成像的可编码基因标签的重要第一步.
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