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转基因小鼠表达人类阿波利波蛋白的异质生成 (a)

R M Lawn¹, D P Wade, R E Hammer

¹Division of Cardiovascular Medicine, Stanford University School of Medicine, California 94305.

|December 17, 1992

概括

升高的脂蛋白 (a) [Lp (a) ]水平增加了动脉样硬化风险. 表达人类阿波利波蛋白的转基因小鼠产生了更多的大动脉病变,证明了Lp在心血管疾病发展中的作用.

科学领域:

心血管生物学心血管生物学
脂质代谢脂质代谢是什么
遗传学是一个遗传学.

背景情况:

血脂蛋白升高 (Lp) 是动脉样硬化,心肌梗塞,中风和静脉缩的重要危险因素.
结构上,Lp (a) 与低密度脂蛋白相似,但包括非脂蛋白 (a),一个等离子体同类物.
Lp(a) 水平在很大程度上是遗传的,并且对传统干预措施具有抵抗力,这使实验研究复杂化.

研究的目的:

研究阿波利波蛋白在动脉样硬化发展中的作用.
建立一个合适的动物模型来研究Lp(a) ]对心血管疾病的影响.

主要方法:

产生表达人类阿波利波蛋白的转基因小鼠.
转基因和对照小鼠的病变发展的比较.
分析阿波利波蛋白在动脉壁中的局部.

主要成果:

与对照组相比,转基因小鼠对主动脉中脂质染色病变的敏感性增加.
发现阿波脂蛋白 (a) 与动脉壁内的脂质沉积共同局部化.

结论:

人类阿波利波蛋白在小鼠中的表达促进了动脉样硬化.
这种转基因模型为研究Lp (a) 对心血管病理学的贡献提供了有价值的工具.

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