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从较低的真核生物中拼接的领导RNA被转接到哺乳动物细胞中

J P Bruzik¹, T Maniatis

¹Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

|December 17, 1992

概括

转接,从单独的分子连接RNA外子,发生在各种生物体中. 这项研究证明了哺乳动物细胞中精确的转接合,揭示了真核生物和哺乳动物之间保存的接合机制.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
在RNA生物学,RNA生物学.

背景情况:

跨拼接,是从单独的RNA分子中连接出异子的过程,在诸如线虫的各种真核生物中已知.
这个过程涉及到一个拼接的领导RNA (SLRNA) 和一个前信使RNA (pre-mRNA).
之前在哺乳动物系统中进行体外转接的尝试显示效率有限,取决于基质互补性.

研究的目的:

调查哺乳动物细胞中转接合的可行性和准确性.
为了确定哺乳动物细胞是否可以在体内和体外进行转接.
探索下层真核生物和哺乳动物之间拼接机械的功能性保护.

主要方法:

利用了人工哺乳动物的mRNA和拼接的领导RNA.
在体外和体外哺乳动物细胞系统中进行过跨拼接实验.
分析了使用线虫和哺乳动物3'拼接点拼接的效率和准确性.

主要成果:

在哺乳动物细胞中,在体外和体内都表现出精确的拼接领导RNA的跨拼接.
表明线虫和哺乳动物的3'拼接位都能在哺乳动物的跨拼接中充当受体.
证实了哺乳动物细胞中转接合的潜力,挑战了以前的限制.

结论:

哺乳动物细胞拥有精确转接的机制.
在较低的真核生物和哺乳动物之间的RNA剪接机制中存在显著的功能性保护.
转接是哺乳动物系统中的一个可行的过程,对基因表达和潜在的治疗应用有影响.

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