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通过高通量合成和实验设计获得的与水相容的分子印记聚合物
Beate Dirion1, Zoe Cobb, Eric Schillinger
1Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg Universität Mainz, Duesbergweg 10-14, 55099 Mainz, Germany.
Journal of the American Chemical Society
|December 5, 2003
概括
一种新的迷你MIP技术能够快速合成和评估用于水性环境的分子印记聚合物. 优化聚合物显示出高选择性和从血中提取布皮瓦卡因的能力.
科学领域:
- 聚合物化学 聚合物化学
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 开发适用于水性环境的选择性吸附剂是一项挑战.
- 分子印记聚合物 (MIP) 具有潜力,但需要针对特定应用进行优化.
- 为了高效的MIP开发,需要高通量方法.
研究的目的:
- 开发一种高吞吐量技术,以缩小规模 (迷你MIP) 合成和评估分子印制聚合物 (MIP).
- 优化MIP用于纯水性环境,特别是局部麻醉剂Bupivacaine.
- 为了评估优化的MIP作为染色体静止相和分析物提取的性能.
主要方法:
- 使用一个4个端口的液体处理机器人以快速分配96个井板格式来合成MIP库.
- 已准备的 80 种聚合物的库 (大约. 每个50毫克) 在24小时内.
- 在批量模式下使用UV单色板阅读器评估MIP重新绑定能力和选择性,在大约1周内完成图书馆评估.
主要成果:
- 优化了布皮瓦卡因在水系统中的MIP,通过不同的共纳米 (HEMA),交叉链接比率和基因.
- 在水中达到高印记因子 (IF),主要是由于与非印记聚合物 (NIP) 的结合减少.
- 证明了强大的模板保留和低的非特异性结合,以优化MIP作为染色体相和血提取.
结论:
- 开发的迷你MIP技术能够快速,高吞吐量合成和评估MIP.
- 优化的MIP在水性环境中表现出色,具有很高的选择性和容量.
- 这些MIP适用于诸如染色分离和从血等复杂矩阵中提取分析物的应用.
相关概念视频
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Step growth polymerization involves bi or multifunctional monomers. Bifunctional monomers react to form linear step growth polymers, whereas multifunctional monomers react to form non-linear or branched polymers.
As the step-growth polymerization involves step-wise condensation of monomers, the molecular weight also builds up eventually. Consequently, high molecular weight polymers are obtained at the late stages of the polymerization, where 99% of monomers have been consumed.
The extent of the...
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