确定Xq和Yq端粒附近的第二个伪自体区域
D Freije1, C Helms, M S Watson
1Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110.
概括
基因交换发生在X和Y染色体之间,它们的端粒. 这项研究确定了第二个伪自体区域,为性别染色体之间的重组提供了证据.
科学领域:
- 人类遗传学 人类遗传学
- 分子生物学分子生物学
- 生殖生物学 生殖生物学
背景情况:
- X和Y染色体 (Xqter和Yqter) 的端粒在半变异过程中结合在一起.
- 超过400千基的序列同质性表明Xqter和Yqter之间的潜在遗传交换.
研究的目的:
- 为了研究Xqter和Yqter之间遗传交换的假设.
- 识别和描述X和Y染色体之间的潜在的伪自体区域.
主要方法:
- 从含有Xqter序列的酵母人工染色体克隆中开发了两个微卫星标记物.
- 在参考血统中追踪这些标记物的遗传 (人类多态学研究中心,巴黎).
- 分析了195个关于重组事件的信息性雄性半导体.
主要成果:
- 在Xqter和Yqter位置之间观察到四个重组事件.
- 在三例中,男性后代继承了父系X等位基因.
- 在一个例子中,一个女性后代继承了她父亲的Y等位基因.
结论:
- 这些数据提供了Xq和Yq端粒之间的遗传交换的有力证据.
- 这种交换定义了人类性染色体上的第二个伪自体区域 (PAR2).
- X和Y染色体之间的重组比以前认为的更广泛.
相关概念视频
Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
¹H NMR Signal Multiplicity: Splitting Patterns
When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
Electric Field of Two Equal and Opposite Charges
Atoms generally contain the same number of positively and negatively charged particles, protons, and electrons. Hence, they are electrically neutral. However, the centers of the positive and negative charges do not always coincide. In such a scenario, the electric field of an atom may not be zero.
A separation of the positive and negative charges can lead to a weak, remnant effect of the positive and negative charges. The expectation is that the more the distance between the positive and...
A separation of the positive and negative charges can lead to a weak, remnant effect of the positive and negative charges. The expectation is that the more the distance between the positive and...
Second Uniqueness Theorem
Consider a region consisting of several individual conductors with a definite charge density in the region between these conductors. The second uniqueness theorem states that if the total charge on each conductor and the charge density in the in-between region are known, then the electric field can be uniquely determined.
In contrast, consider that the electric field is non-unique and apply Gauss's law in divergence form in the region between the conductors and the integral form to the surface...
In contrast, consider that the electric field is non-unique and apply Gauss's law in divergence form in the region between the conductors and the integral form to the surface...
Area Between Curves: Integrating With Respect to y
Consider a planar region bounded by two curves that are both written as functions of the vertical variable, y. The left and right boundary curves are continuous between y = c and y = d, and these two horizontal lines define the vertical limits of the region. Because the boundaries depend on y rather than x, the area is most appropriately evaluated using horizontal slices.The area is obtained using the Riemann sum method. The region is divided into many thin horizontal strips, each having an...
Quadric Surfaces
Quadric surfaces are three-dimensional surfaces characterized by second-degree equations in the variables x, y, and z. These surfaces are smooth and continuous, and specific combinations of squared and linear terms define their shapes. The main types of quadric surfaces include ellipsoids, cones, paraboloids, and hyperboloids. Each type exhibits distinct geometric features depending on how the variables are arranged and related within the equation.Ellipsoids are closed surfaces formed when all...


