融化合作性的传播沿着DNA的基体脊柱传播
Jessica Becaud1, Isabelle Pompizi, Christian J Leumann
1Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
Journal of the American Chemical Society
|December 11, 2003
概括
DNA 基基骨干转移螺旋域之间的融化合作. 骨干单元的刚性和不对称的分布显著影响了这种结构通信距离.
科学领域:
- 分子生物学分子生物学
- 生物物理学的生物物理.
- 结构生物学 结构生物学
背景情况:
- DNA的基脊柱对其结构完整性和功能至关重要.
- 融化的合作性,即DNA的协调解,对于复制和转录等过程至关重要.
- 了解结构信息是如何沿着DNA骨干传输的,是解读DNA动态的关键.
研究的目的:
- 为了研究DNA基体骨干在远距离螺旋域之间的结构通信中介的作用.
- 确定骨干的修改,特别是核酸单元的修改如何影响化合作的转移.
- 探索骨干单位灵活性和分布对合作转移程度的影响.
主要方法:
- 使用螺旋-凸起-螺旋 DNA 模型系统.
- 在膨胀中嵌入了结构灵活的 (propanediol) 和受约束的 (双循环) 无核酸基骨干单元.
- 这些无核酸单元在DNA链中的数量和分布 (对称与不对称) 变化.
主要成果:
- 通过融化合作性转移来衡量结构性沟通,大约跨越12-20个DNA骨干单位.
- 即使在只有一个DNA链上存在无核酸单元时,也会发生合作性转移的破坏.
- 最长的核糖体插入的长度决定了合作性转移的程度.
- 骨干单位的结构刚度增加,特别是当它们分布不对称时,增加了合作性转移的距离.
结论:
- DNA 基骨干作为一个管道,在螺旋域之间传输结构信息,比如化合作,作为一个管道.
- 骨干修改的长度和形状特性显著调节这种通信的效率和距离.
- 对DNA骨干的不对称修改可以严重破坏合作相互作用,突出显示了序列和结构对称性的重要性.
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01:44Complementary DNA
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02:05DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
02:08Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...