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相关概念视频

Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

探测芯片上α-elastin水性双相系统形成的机制.

Yanjie Zhang1, Hanbin Mao, Paul S Cremer

  • 1Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.

Journal of the American Chemical Society
|December 11, 2003
PubMed
概括

研究了对α-elastin的水性双相系统 (ATPS) 形成动力学. 速率常数随着蛋白质度的增加而增加,这表明凝聚机制,激活能量较低.

科学领域:

  • 生物物理学的生物物理.
  • 材料科学 材料科学 材料科学
  • 蛋白质化学 蛋白质化学

背景情况:

  • 水性双相系统 (ATPS) 对于蛋白质分离和净化至关重要.
  • 了解ATPS形成的动力学对于优化生物过程至关重要.
  • 阿尔法-弹性素的相分离行为为蛋白质自我组装提供了洞察力.

研究的目的:

  • 为了研究α-elastin水性双相系统 (ATPS) 形成的动力学.
  • 阐明ATPS形成的基本机制,特别是凝聚和奥斯瓦尔德成熟.
  • 为了确定与不同的ATPS形成路径相关的激活能量.

主要方法:

  • 使用暗场显微镜与芯片上的线性温度梯度.
  • 记录了蛋白质溶液的散射强度作为温度和时间的函数.
  • 分析了动力学数据,以确定不同蛋白质度的反应顺序和速率常数.

主要成果:

  • 形成ATPS遵循的是第一阶段的过程,速度常数随蛋白质度的增加而增加.
  • 凝聚机制表现出9.5 +/- 0.5 kcal/mol的激活能量.
  • 在二硫酸盐的存在下,奥斯瓦尔德成熟占主导地位,将激活能量增加到33 +/- 2 kcal/mol,并产生二次动力学.

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Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

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Last Updated: Jul 7, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
10:01

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

结论:

  • 这项研究揭示了阿尔法-弹性素ATPS形成的独特动态路径,由凝聚或奥斯瓦尔德成熟控制.
  • 蛋白质度通过凝聚直接影响ATPS形成的速度.
  • 这些发现为控制和优化基于蛋白质的ATPS应用提供了关键数据.