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从胚胎干细胞中衍生出胚胎生殖细胞和雄性结合体
Niels Geijsen1, Melissa Horoschak, Kitai Kim
1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
Nature
|December 12, 2003
概括
研究人员成功地在实验室中从胚胎干细胞中衍生出小鼠卵和精子细胞 (胚胎细胞). 这一突破为研究生殖线发育和表观遗传学提供了一个新的模型.
科学领域:
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
- 生殖生物学 生殖生物学
背景情况:
- 哺乳动物的配体起源于胚胎发育早期建立的原始生殖细胞.
- 原始生殖细胞从近端表皮质细胞发育,也为早期的血统做出贡献.
- 胚胎干细胞在体外形成胚胎体,模仿早期胚胎组织并支持血液发育.
研究的目的:
- 调查胚胎体是否可以支持原始生殖细胞的形成和发育.
- 从胚胎体中建立不断增长的胚胎生殖细胞系.
- 探索这个系统在研究生殖系表观遗传学和生殖系生成方面的潜力.
主要方法:
- 在体外分化小鼠胚胎干细胞以形成胚胎体.
- 从胚胎体中分离出来的原始生殖细胞.
- 培养和表征衍生的胚胎生殖细胞,评估表观遗传标记物和伴生体潜力.
主要成果:
- 成功地从胚胎体中分离出原始的生殖细胞.
- 建立了不断增长的胚胎生殖细胞系.
- 在胚胎生殖细胞中,证明了甲基化印记 (Igf2r,H19) 的擦除,这是生殖系特征.
- 表明胚胎体支持原始生殖细胞成熟成为功能性平性雄性性交配体.
- 证实,这些衍生性雌激细胞可以恢复二分化并支持卵细胞注射后的胚胎细胞发育.
结论:
- 胚胎体提供了一个可行的体外系统,可以从胚胎干细胞生成生殖细胞.
- 这种系统有助于研究生殖线表观遗传修饰和哺乳动物体育生成.
- 衍生的胚胎生殖细胞表现出关键的生殖系特征和发育潜力.
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