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Updated: Jul 15, 2026

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
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Published on: April 27, 2010

一种化学酶方法,用于快速和灵敏地检测O-GlcNAc的翻译后修饰

Nelly Khidekel1, Sabine Arndt, Nathan Lamarre-Vincent

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Journal of the American Chemical Society
|December 25, 2003
PubMed
概括

一种新的化学酶策略使得O-GlcNAc转化后修饰的敏感检测成为可能. 这种方法使用工程酶以子标记蛋白质,允许生物素附着和化学发光检测.

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相关概念视频

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...