在新型pH触发自组装β片带中的能量迁移
Veysel Kayser1, David A Turton, Amalia Aggeli
1Department of Chemistry and Centre for Chemical Dynamics, University of Leeds, Leeds, LS2 9JT, UK.
Journal of the American Chemical Society
|January 8, 2004
概括
在自组装的酸带中,证明了酸残留物之间的能量迁移. 这种现象对于理解的行为至关重要,它发生在低pH条件下形成的稳定带中.
科学领域:
- 生物物理学的生物物理.
- 超分子化学 超分子化学
- 蛋白质科学 蛋白质科学
背景情况:
- 自组合的结构在生物系统中至关重要.
- 托芬 (Trp) 光对其微环境敏感.
- 了解中的能量转移,可以告知蛋白质的折叠和功能.
研究的目的:
- 通过实验来证明,在自组装的酸带中,托残留物之间的能量迁移.
- 用pH来描述组合的结构和动态变化.
- 调查当地环境对托芬光异构的作用.
主要方法:
- 光光谱学 (光谱,火,异性变异).
- 11-氨基酸的pH依赖的自我组装,Trp在6.位置.
- 在水和糖糖粘度混合物中测量时间分辨率光异质性.
- 主方程是Trp-Trp能量迁移的动态建模.
主要成果:
- 体自组装成带是对pH值敏感的,在低pH值创建一个疏水的内部.
- 托芬光寿命在低pH下较短,这表明火.
- 时间解析的光异性变异性显示在水中组装时的变化很小.
- 在高pH的粘性介质中, anisotropy衰变显著增加,但在低pH时没有.
- 低pH值下的脱极化归因于能量迁移,而不是旋转扩散.
结论:
- 直接实验证据表明,在自组装的皮带中,托残留物之间存在能量迁移.
- 片带在低pH值下具有稳定,疏水的内部.
- 能量迁移,而不是旋转扩散,解释了低pH下光脱极化.
- 主方程建模支持提议的Trp-Trp能量迁移机制.
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