在Arabidopsis thaliana中,Vernalization是由PHD指蛋白VIN3介导的
Sibum Sung1, Richard M Amasino
1Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA.
Nature
|January 9, 2004
概括
维纳化是一个需要长时间的寒冷的过程,通过稳定地抑制FLC基因,使得像Arabidopsis thaliana这样的植物能够开花. 一个新发现的基因测量了寒冷的持续时间,并通过基因素修改建立了这一关键的本地化状态.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在两年生和冬季一年生中,在第一个生长季节内抑制了开花.
- 化,暴露于冬季长时间的寒冷,对于克服这种开花障碍至关重要.
- 在Arabidopsis thaliana中,花C (FLC) 基因充当了花抑制剂,高表达阻止了第一季的开花.
研究的目的:
- 为了识别参与测量寒冷持续时间的基因.
- 了解建立本土化状态的分子机制.
- 阐明 vernalisation 如何导致稳定的 FLC 基因抑制.
主要方法:
- 在Arabidopsis thaliana中进行遗传选,以识别具有改变本地化反应的突变物.
- 对基因表达的分子分析,专注于FLC.
- 染色体免疫沉 (ChIP) 用于研究FLC位点上的基因组修饰.
主要成果:
- 鉴定了一种新型基因,该基因对于感知寒冷的持续时间和启动本地化至关重要.
- 证明本地化诱导了FLC的线粒细胞稳定抑制.
- 证据表明,FLC基因沉默在本地化过程中涉及到特定的变化在基因组修改.
结论:
- 一个新发现的基因在植物对冬季寒冷持续时间的感知中起着关键作用.
- 民族化在FLC位点建立了可遗传的表观遗传状态,确保了稳定的抑制.
- 基质子修饰是关键的分子事件,调解了FLC的本地化诱导的沉默.
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