树的发育受到CREST的调节,这是一个由调节的转录激活剂
Hiroyuki Aizawa1, Shu-Ching Hu, Kathryn Bobb
1Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
概括
响应的交换激活器,CREST,对于大脑发育至关重要. 它调节基因表达和神经元生长,基因干扰导致树发育缺陷.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 发育生物学 发展生物学
背景情况:
- 神经元活动通过依赖的基因表达对大脑发育有深远的影响.
- 了解将神经元活动与发育变化联系起来的分子机制至关重要.
研究的目的:
- 识别和描述介于发育中的大脑中依赖基因表达的新型因素.
- 为了研究反应性交互激活器CREST在神经元发育中的作用.
主要方法:
- 采用了交叉激活器陷策略来克隆对有反应的交叉激活器.
- 生成和分析的小鼠具有峰基因的有针对性的破坏.
- 从野生型和突变小鼠的皮质神经元中检查了树突发育.
主要成果:
- 成功克隆了一种新型的反应性交换激活器,CREST,一种与SYT相关的核蛋白.
- CREST与cAMP反应元素结合蛋白 (CREB) - 结合蛋白 (CBP) 相互作用,并在发育中的大脑中表达.
- 缺少功能基因的小鼠表现出皮层和海马体状状体发育受损,皮层神经元中依赖的状体生长受损.
结论:
- 通过CREST介导的转录的激活是神经元形态发生的关键调节者.
- 在大脑发育过程中,CREST在神经元的结构发展中起着至关重要的作用.
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Transcription Can Produce Different Kinds...
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Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
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Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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