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相关概念视频

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

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相关实验视频

Updated: Jul 1, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

酵母中未结合的mRNAs的核保留是由周核Mlp1介导的.

Vincent Galy1, Olivier Gadal, Micheline Fromont-Racine

  • 1Unité de Biologie Cellulaire du Noyau, CNRS URA 2582, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France.

Cell
|January 14, 2004
PubMed
概括
此摘要是机器生成的。

酵母中含有内子的mRNAs的核保留是由周核蛋白Mlp1蛋白调节的. 删除MLP1减少了保留,这表明MLP1作为核质量控制机制用于mRNA出口.

更多相关视频

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 15, 2014

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

相关实验视频

Last Updated: Jul 1, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
14:44

Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

Published on: March 15, 2014

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
08:00

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 酵母遗传学 酵母遗传学

背景情况:

  • 内子含有mRNA的核保留机制尚不清楚.
  • 了解mRNA处理和输出对于基因表达调节至关重要.

研究的目的:

  • 为了阐明酵母中含有内子的mRNA核保留的分子机制.
  • 为了确定参与调节mRNA出口的关键蛋白质.

主要方法:

  • 在酵母中进行基因删除研究 (MLP1,RRP6).
  • 对mRNA拼接和核保留的分析.
  • 对拼接突变 (ts-prp18 delta) 的研究.
  • 对Mlp1蛋白的局部化研究.

主要成果:

  • 周核Mlp1调解了含内子mRNAs的保留.
  • 删除MLP1会影响保留,但不会影响拼接效率.
  • 在结合突变体和核外体功能受到损害时,依赖Mlp1的RNA泄漏会加剧.
  • 保留与5'拼接部位相关,Mlp1定位于与染色质相关的核包膜区域.

结论:

  • 周核Mlp1作为一种关键的质量控制因素,在出口前在核包裹中保留有缺陷的前mRNA.
  • 这种机制确保了mRNA出口和随后的蛋白质合成的忠实性.