在哺乳动物细胞的线粒分裂过程中,戈尔吉膜仍然与内细胞网隔离
Matt Yasuo Pecot1, Vivek Malhotra
1Cell and Developmental Biology Department, University of California San Diego, La Jolla, CA 92093, USA.
Cell
|January 14, 2004
概括
戈尔吉膜在哺乳动物细胞的线粒分裂过程中不会与内细胞网 (ER) 融合. 这项研究使用了一种新的拉巴胺素诱导近距离测试来追踪戈尔吉蛋白,发现它们在整个细胞分裂过程中与ER保持鲜明的区别.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 有机体动力学 有机体动力学
背景情况:
- 细胞分裂期间的器官命运对于细胞分裂至关重要.
- 在线粒分裂过程中,戈尔吉和内细胞网膜 (ER) 之间的关系尚不清楚.
- 了解器官分离可以确保细胞在线粒分裂后的正常细胞功能.
研究的目的:
- 为了调查戈尔吉膜是否在线粒分裂过程中与ER融合.
- 为了确定戈尔吉蛋白是否在细胞分裂过程中与ER蛋白相互作用.
- 为了阐明高尔基和ER在哺乳动物细胞分裂过程中的分离机制.
主要方法:
- 在COS细胞中开发了一种使用FKBP标记的Golgi蛋白和ER保留的FRAP蛋白的近距离测试.
- 使用拉巴胺来诱导FKBP和FRAP之间的关联,如果它们相互作用,就会捕获蛋白质.
- 在rapamycin的存在下,在线解后监测了Golgi蛋白的局部化.
主要成果:
- 拉帕米辛治疗没有导致FKBP融合的Golgi蛋白在ER中被保留.
- 戈尔吉蛋白质始终在新形成的戈尔吉堆中发现,而不是ER.
- 证明Golgi膜在线粒分裂过程中与ER保持物理分离.
结论:
- 戈尔吉膜在哺乳动物细胞的线粒分裂过程中保持了与ER的独特身份.
- 这项研究提供了直接的证据,反对Golgi-ER融合在线粒分裂过程中.
- 建立了一种可靠的方法来研究细胞分裂期间的有机细胞膜相互作用.
相关概念视频
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:12Golgi Matrix Proteins
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
02:33Distribution of Cytoplasmic Content
Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...
01:26Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...